Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 5395366..5395984 Replicon chromosome
Accession NZ_CP080139
Organism Escherichia coli strain PK8241

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZY05_RS27130 Protein ID WP_001291435.1
Coordinates 5395366..5395584 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZY05_RS27135 Protein ID WP_000344800.1
Coordinates 5395610..5395984 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY05_RS27095 (5390655) 5390655..5391227 + 573 WP_000779831.1 YbaY family lipoprotein -
KZY05_RS27100 (5391258) 5391258..5391569 - 312 WP_000409911.1 MGMT family protein -
KZY05_RS27110 (5391948) 5391948..5392301 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KZY05_RS27115 (5392343) 5392343..5393893 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZY05_RS27120 (5394057) 5394057..5394527 - 471 WP_000136192.1 YlaC family protein -
KZY05_RS27125 (5394643) 5394643..5395194 - 552 WP_000102564.1 maltose O-acetyltransferase -
KZY05_RS27130 (5395366) 5395366..5395584 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KZY05_RS27135 (5395610) 5395610..5395984 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZY05_RS27140 (5396530) 5396530..5399679 - 3150 WP_069723301.1 efflux RND transporter permease AcrB -
KZY05_RS27145 (5399702) 5399702..5400895 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T211047 WP_001291435.1 NZ_CP080139:c5395584-5395366 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T211047 NZ_CP104667:52764-53144 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCTGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAACTATATGGTTCCAATATTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT211047 WP_000344800.1 NZ_CP080139:c5395984-5395610 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT211047 NZ_CP104667:52543-52764 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CCGAATTTGCATCCCTGTTTGACACTCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References