Detailed information of TA system TA211046

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5189242..5190077
• Replicon: chromosome
• Accession: NZ_CP080139
• Organism: Escherichia coli strain PK8241

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: KZY05_RS26130
• Protein ID: WP_000854821.1
• Coordinates: 5189700..5190077 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0D404
• Locus tag: KZY05_RS26125
• Protein ID: WP_024214935.1
• Coordinates: 5189242..5189610 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY05_RS26100 (5186644)	5186644..5186877	+	234	WP_001117566.1	DUF905 family protein	-
KZY05_RS26105 (5186967)	5186967..5187785	+	819	WP_001234397.1	DUF932 domain-containing protein	-
KZY05_RS26110 (5187877)	5187877..5188362	+	486	WP_024214936.1	antirestriction protein	-
KZY05_RS26115 (5188378)	5188378..5188854	+	477	WP_001186745.1	RadC family protein	-
KZY05_RS26120 (5188941)	5188941..5189162	+	222	WP_000692350.1	DUF987 domain-containing protein	-
KZY05_RS26125 (5189242)	5189242..5189610	+	369	WP_024214935.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZY05_RS26130 (5189700)	5189700..5190077	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KZY05_RS26135 (5190074)	5190074..5190271	+	198	Protein_5156	DUF5983 family protein	-
KZY05_RS26140 (5190299)	5190299..5190496	+	198	WP_089075429.1	DUF957 domain-containing protein	-
KZY05_RS26145 (5190581)	5190581..5191429	+	849	WP_001280470.1	DUF4942 domain-containing protein	-
KZY05_RS26150 (5191522)	5191522..5192841	-	1320	WP_000144688.1	site-specific integrase	-
KZY05_RS26155 (5193219)	5193219..5194433	+	1215	WP_000772643.1	tyrosine-type recombinase/integrase	-
KZY05_RS26160 (5194862)	5194862..5195065	+	204	WP_000035054.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5183720..5208423	24703

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T211046 WP_000854821.1 NZ_CP080139:5189700-5190077 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T211046 NZ_CP104667:29906-30208 [Escherichia coli]
ATGAACAAAAAGATTAACATAAAGAATTTCAGGGATGAGTGGCTTGATGATTTTTTTGAATTTTCAATACCACACAAAAA
GATACCTTCTGATATTCAGATTACATTGTTGCGGAAGCTGGATATTATCAATGCTGCAACTACTTGGAAGGACTTGAGAT
CACCACCAGGTAATCGGTATGAGGAATTGTCAGGGAAGCTAAACGGCTATTCATCAATAAGGGTGAATAAGCAATACAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13605.41 Da
• Isoelectric Point: 6.4659

>AT211046 WP_024214935.1 NZ_CP080139:5189242-5189610 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT211046 NZ_CP104667:30280-30618 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACTGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTTAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAACAATCGTAAGCTAACAATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGACCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54

Antitoxin

• AlphaFold DB: A0A1M0D404