Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 4915140..4915741 Replicon chromosome
Accession NZ_CP080139
Organism Escherichia coli strain PK8241

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag KZY05_RS24740 Protein ID WP_001216034.1
Coordinates 4915140..4915520 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KZY05_RS24745 Protein ID WP_001190712.1
Coordinates 4915520..4915741 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY05_RS29530 (4910245) 4910245..4910358 - 114 WP_075362742.1 ash family protein -
KZY05_RS24715 (4910581) 4910581..4912065 - 1485 WP_000124150.1 terminase -
KZY05_RS24720 (4912065) 4912065..4913258 - 1194 WP_069723424.1 terminase -
KZY05_RS24725 (4913344) 4913344..4913796 - 453 WP_001326849.1 late promoter-activating protein -
KZY05_RS24730 (4913885) 4913885..4914928 - 1044 WP_069723425.1 DUF968 domain-containing protein -
KZY05_RS24735 (4914956) 4914956..4915135 - 180 WP_001339207.1 hypothetical protein -
KZY05_RS24740 (4915140) 4915140..4915520 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KZY05_RS24745 (4915520) 4915520..4915741 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KZY05_RS24750 (4915814) 4915814..4916203 - 390 WP_000506730.1 S24 family peptidase -
KZY05_RS24755 (4916378) 4916378..4916950 + 573 WP_069723426.1 DNA-binding protein -
KZY05_RS24760 (4916957) 4916957..4917208 - 252 WP_069723427.1 DNA polymerase III subunit theta -
KZY05_RS24765 (4917658) 4917658..4917795 + 138 WP_000123562.1 hypothetical protein -
KZY05_RS24770 (4917870) 4917870..4918232 - 363 WP_001261544.1 hypothetical protein -
KZY05_RS24775 (4918229) 4918229..4919104 - 876 WP_232380429.1 hypothetical protein -
KZY05_RS29535 (4919081) 4919081..4919215 - 135 Protein_4887 hypothetical protein -
KZY05_RS24780 (4919549) 4919549..4919727 - 179 Protein_4888 hypothetical protein -
KZY05_RS24785 (4919729) 4919729..4920109 - 381 WP_232380430.1 ead/Ea22-like family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island dfrA12 / aadA2 / qacE / sul1 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id - 4882586..4977485 94899


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T211043 WP_001216034.1 NZ_CP080139:c4915520-4915140 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T211043 NZ_CP104666:c43449-43300 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT211043 WP_001190712.1 NZ_CP080139:c4915741-4915520 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT211043 NZ_CP104666:43493-43554 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References