Detailed information of TA system TA211021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1126729..1127255
• Replicon: chromosome
• Accession: NZ_CP080139
• Organism: Escherichia coli strain PK8241

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: KZY05_RS06030
• Protein ID: WP_000323025.1
• Coordinates: 1126968..1127255 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: KZY05_RS06025
• Protein ID: WP_000534858.1
• Coordinates: 1126729..1126968 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY05_RS05995 (1121998)	1121998..1123272	+	1275	WP_075362599.1	ISNCY family transposase	-
KZY05_RS29295 (1124544)	1124544..1124651	+	108	Protein_1180	protein YdfW	-
KZY05_RS06005 (1125088)	1125088..1125342	-	255	Protein_1181	FlxA-like family protein	-
KZY05_RS06015 (1126110)	1126110..1126196	-	87	Protein_1183	protein FlxA	-
KZY05_RS06020 (1126399)	1126399..1126704	-	306	WP_071600497.1	protein YdfV	-
KZY05_RS06025 (1126729)	1126729..1126968	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
KZY05_RS06030 (1126968)	1126968..1127255	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
KZY05_RS06035 (1127327)	1127327..1127482	+	156	WP_000813269.1	type I toxin-antitoxin system toxin HokD	-
KZY05_RS06040 (1127951)	1127951..1128550	+	600	WP_025670565.1	DUF1367 family protein	-
KZY05_RS06045 (1128672)	1128672..1129694	+	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
KZY05_RS06050 (1129691)	1129691..1130488	+	798	WP_075362601.1	IS21-like element IS100kyp family helper ATPase IstB	-
KZY05_RS06060 (1131855)	1131855..1132112	+	258	WP_069723324.1	DUF1364 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1095514..1168555	73041
	-	inside	IScluster/Tn	-	-	1118394..1131704	13310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T211021 WP_000323025.1 NZ_CP080139:1126968-1127255 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T211021 NZ_CP104665:163886-164071 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT211021 WP_000534858.1 NZ_CP080139:1126729-1126968 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT211021 NZ_CP104665:164088-164498 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGCCGCGCACTTCGAAACCTTATGCGAGATGG
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8