Detailed information of TA system TA211016

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 479162..479997
• Replicon: chromosome
• Accession: NZ_CP080139
• Organism: Escherichia coli strain PK8241

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1L6W3P3
• Locus tag: KZY05_RS02435
• Protein ID: WP_075362639.1
• Coordinates: 479620..479997 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L6W4Z7
• Locus tag: KZY05_RS02430
• Protein ID: WP_070749977.1
• Coordinates: 479162..479530 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY05_RS02390 (474405)	474405..474944	+	540	WP_001104026.1	DUF4339 domain-containing protein	-
KZY05_RS02395 (475214)	475214..475627	+	414	WP_000789536.1	hypothetical protein	-
KZY05_RS02400 (475766)	475766..475944	-	179	Protein_466	hypothetical protein	-
KZY05_RS02405 (476038)	476038..476856	+	819	WP_075362641.1	DUF932 domain-containing protein	-
KZY05_RS02410 (477198)	477198..477671	+	474	WP_075362640.1	antirestriction protein	-
KZY05_RS02415 (477687)	477687..478163	+	477	WP_001186770.1	RadC family protein	-
KZY05_RS02420 (478232)	478232..478453	+	222	WP_000692312.1	DUF987 domain-containing protein	-
KZY05_RS02425 (478468)	478468..479112	+	645	WP_039025881.1	hypothetical protein	-
KZY05_RS02430 (479162)	479162..479530	+	369	WP_070749977.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZY05_RS02435 (479620)	479620..479997	+	378	WP_075362639.1	TA system toxin CbtA family protein	Toxin
KZY05_RS02440 (479994)	479994..480482	+	489	WP_039022338.1	DUF5983 family protein	-
KZY05_RS02445 (480494)	480494..480691	+	198	WP_072643848.1	DUF957 domain-containing protein	-
KZY05_RS02450 (480776)	480776..481618	+	843	WP_075362638.1	DUF4942 domain-containing protein	-
KZY05_RS02460 (482108)	482108..482326	-	219	WP_001040187.1	translation initiation factor IF-1	-
KZY05_RS02465 (482611)	482611..483315	-	705	WP_001241676.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14112.20 Da
• Isoelectric Point: 8.2962

>T211016 WP_075362639.1 NZ_CP080139:479620-479997 [Escherichia coli]
MKTLPDTHVREVSRCPSPVTIWKTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T211016 NZ_CP104660:c4820037-4819753 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13860.63 Da
• Isoelectric Point: 6.3177

>AT211016 WP_070749977.1 NZ_CP080139:479162-479530 [Escherichia coli]
VSDTFHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQVFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT211016 NZ_CP104660:c4820269-4820027 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAACAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A1L6W3P3

Antitoxin

• AlphaFold DB: A0A1L6W4Z7