Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-DnaT |
| Location | 32777..33299 | Replicon | plasmid pPK8277-49kb |
| Accession | NZ_CP080137 | ||
| Organism | Escherichia coli strain PK8277 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G3CAN5 |
| Locus tag | KZY04_RS23940 | Protein ID | WP_000220560.1 |
| Coordinates | 32777..33058 (-) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | G3CAG1 |
| Locus tag | KZY04_RS23945 | Protein ID | WP_000121743.1 |
| Coordinates | 33048..33299 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KZY04_RS23895 (KZY04_23920) | 27786..28277 | - | 492 | WP_000872475.1 | transcription termination/antitermination NusG family protein | - |
| KZY04_RS23900 (KZY04_23925) | 28638..29795 | - | 1158 | WP_000538023.1 | DNA distortion polypeptide 2 | - |
| KZY04_RS23905 (KZY04_23930) | 29798..30343 | - | 546 | WP_038976855.1 | DNA distortion polypeptide 1 | - |
| KZY04_RS23910 (KZY04_23935) | 30670..30942 | - | 273 | WP_000160399.1 | hypothetical protein | - |
| KZY04_RS23915 (KZY04_23940) | 30955..31104 | - | 150 | WP_000003880.1 | hypothetical protein | - |
| KZY04_RS23920 (KZY04_23945) | 31391..31720 | - | 330 | WP_000866648.1 | hypothetical protein | - |
| KZY04_RS23925 (KZY04_23950) | 31813..32028 | - | 216 | WP_001180116.1 | hypothetical protein | - |
| KZY04_RS23930 (KZY04_23955) | 32018..32263 | - | 246 | WP_000356546.1 | hypothetical protein | - |
| KZY04_RS23935 (KZY04_23960) | 32308..32631 | - | 324 | WP_001181903.1 | hypothetical protein | - |
| KZY04_RS23940 (KZY04_23965) | 32777..33058 | - | 282 | WP_000220560.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KZY04_RS23945 (KZY04_23970) | 33048..33299 | - | 252 | WP_000121743.1 | plasmid stabilization protein | Antitoxin |
| KZY04_RS23950 (KZY04_23975) | 34555..35391 | + | 837 | WP_001050931.1 | RepB family plasmid replication initiator protein | - |
| KZY04_RS23955 (KZY04_23980) | 35431..35877 | + | 447 | WP_001074386.1 | hypothetical protein | - |
| KZY04_RS23960 (KZY04_23985) | 36014..36367 | + | 354 | WP_000876434.1 | DNA distortion polypeptide 3 | - |
| KZY04_RS23965 (KZY04_23990) | 36427..37395 | - | 969 | WP_000654804.1 | IS5-like element IS903B family transposase | - |
| KZY04_RS23970 (KZY04_23995) | 37397..37690 | - | 294 | WP_015060510.1 | hypothetical protein | - |
| KZY04_RS23975 (KZY04_24000) | 37706..37936 | - | 231 | WP_000051064.1 | plasmid partition protein ParG | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | qnrS1 / aph(3')-Ia / blaTEM-215 / tet(A) | - | 1..49201 | 49201 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11005.83 Da Isoelectric Point: 10.5938
>T211015 WP_000220560.1 NZ_CP080137:c33058-32777 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
Download Length: 282 bp
>T211015 NZ_CP104660:c4704815-4704282 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTATTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTATTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9194.43 Da Isoelectric Point: 4.1683
>AT211015 WP_000121743.1 NZ_CP080137:c33299-33048 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
Download Length: 252 bp
>AT211015 NZ_CP104660:c4705092-4704826 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G3CAN5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3S5I301 |