Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/- |
| Location | 8899..9491 | Replicon | plasmid pPK8277-MCR1 |
| Accession | NZ_CP080136 | ||
| Organism | Escherichia coli strain PK8277 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | A0A1I9ZJC5 |
| Locus tag | KZY04_RS23445 | Protein ID | WP_000513532.1 |
| Coordinates | 8899..9147 (+) | Length | 83 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | A0A1I9ZJ49 |
| Locus tag | KZY04_RS23450 | Protein ID | WP_000681609.1 |
| Coordinates | 9144..9491 (+) | Length | 116 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KZY04_RS23410 (KZY04_23425) | 3911..4576 | - | 666 | WP_000532149.1 | division plane positioning ATPase MipZ | - |
| KZY04_RS23415 (KZY04_23430) | 5165..5494 | + | 330 | WP_001542022.1 | hypothetical protein | - |
| KZY04_RS23420 (KZY04_23435) | 5895..6410 | + | 516 | WP_001025393.1 | J domain-containing protein | - |
| KZY04_RS23425 (KZY04_23440) | 6514..7056 | + | 543 | WP_001083904.1 | hypothetical protein | - |
| KZY04_RS23430 (KZY04_23445) | 7053..7307 | + | 255 | WP_001230709.1 | hypothetical protein | - |
| KZY04_RS23435 (KZY04_23450) | 7304..7546 | + | 243 | WP_000151456.1 | hypothetical protein | - |
| KZY04_RS23440 (KZY04_23455) | 8117..8410 | + | 294 | WP_000673428.1 | hypothetical protein | - |
| KZY04_RS23445 (KZY04_23460) | 8899..9147 | + | 249 | WP_000513532.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| KZY04_RS23450 (KZY04_23465) | 9144..9491 | + | 348 | WP_000681609.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| KZY04_RS24380 | 9579..10022 | - | 444 | WP_225930715.1 | hypothetical protein | - |
| KZY04_RS23460 (KZY04_23475) | 10263..10562 | + | 300 | WP_034169410.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| KZY04_RS23465 (KZY04_23480) | 10822..11148 | - | 327 | WP_000206697.1 | hypothetical protein | - |
| KZY04_RS23470 (KZY04_23485) | 11150..11473 | - | 324 | WP_223666488.1 | hypothetical protein | - |
| KZY04_RS24385 | 11450..11590 | - | 141 | Protein_19 | hypothetical protein | - |
| KZY04_RS23475 (KZY04_23490) | 11610..12317 | - | 708 | WP_172690216.1 | hypothetical protein | - |
| KZY04_RS23480 (KZY04_23495) | 12319..13071 | - | 753 | WP_000797966.1 | conjugal transfer protein TraL | - |
| KZY04_RS23485 (KZY04_23500) | 13089..13490 | - | 402 | WP_021575837.1 | hypothetical protein | - |
| KZY04_RS23490 (KZY04_23505) | 13859..14200 | + | 342 | WP_000730722.1 | plasmid mobilization relaxosome protein MobC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | mcr-1.1 | - | 1..60961 | 60961 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 83 a.a. Molecular weight: 9378.03 Da Isoelectric Point: 10.4800
>T211014 WP_000513532.1 NZ_CP080136:8899-9147 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
Download Length: 249 bp
>T211014 NZ_CP104660:4107046-4107264 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA
Antitoxin
Download Length: 116 a.a. Molecular weight: 13206.97 Da Isoelectric Point: 4.8313
>AT211014 WP_000681609.1 NZ_CP080136:9144-9491 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
Download Length: 348 bp
>AT211014 NZ_CP104660:4106645-4107019 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1I9ZJC5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1I9ZJ49 |