211013

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 7053..7546 Replicon plasmid pPK8277-MCR1
Accession NZ_CP080136
Organism Escherichia coli strain PK8277

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag KZY04_RS23430 Protein ID WP_001230709.1
Coordinates 7053..7307 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag KZY04_RS23435 Protein ID WP_000151456.1
Coordinates 7304..7546 (+) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY04_RS24375 2570..2722 + 153 Protein_2 DUF5431 family protein -
KZY04_RS23395 (KZY04_23410) 2667..2789 + 123 WP_223685802.1 Hok/Gef family protein -
KZY04_RS23400 (KZY04_23415) 2939..3535 + 597 WP_000125165.1 ProQ/FINO family protein -
KZY04_RS23405 (KZY04_23420) 3553..3837 - 285 WP_001162371.1 hypothetical protein -
KZY04_RS23410 (KZY04_23425) 3911..4576 - 666 WP_000532149.1 division plane positioning ATPase MipZ -
KZY04_RS23415 (KZY04_23430) 5165..5494 + 330 WP_001542022.1 hypothetical protein -
KZY04_RS23420 (KZY04_23435) 5895..6410 + 516 WP_001025393.1 J domain-containing protein -
KZY04_RS23425 (KZY04_23440) 6514..7056 + 543 WP_001083904.1 hypothetical protein -
KZY04_RS23430 (KZY04_23445) 7053..7307 + 255 WP_001230709.1 hypothetical protein Toxin
KZY04_RS23435 (KZY04_23450) 7304..7546 + 243 WP_000151456.1 hypothetical protein Antitoxin
KZY04_RS23440 (KZY04_23455) 8117..8410 + 294 WP_000673428.1 hypothetical protein -
KZY04_RS23445 (KZY04_23460) 8899..9147 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
KZY04_RS23450 (KZY04_23465) 9144..9491 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
KZY04_RS24380 9579..10022 - 444 WP_225930715.1 hypothetical protein -
KZY04_RS23460 (KZY04_23475) 10263..10562 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
KZY04_RS23465 (KZY04_23480) 10822..11148 - 327 WP_000206697.1 hypothetical protein -
KZY04_RS23470 (KZY04_23485) 11150..11473 - 324 WP_223666488.1 hypothetical protein -
KZY04_RS24385 11450..11590 - 141 Protein_19 hypothetical protein -
KZY04_RS23475 (KZY04_23490) 11610..12317 - 708 WP_172690216.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..60961 60961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-100)

Antitoxin

(38-88)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T211013 WP_001230709.1 NZ_CP080136:7053-7307 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T211013 NZ_CP104660:c3976867-3976550 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT211013 WP_000151456.1 NZ_CP080136:7304-7546 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT211013 NZ_CP104660:c3976557-3976270 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0


Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References