211011

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 19332..19933 Replicon plasmid pPK8277-98kb
Accession NZ_CP080135
Organism Escherichia coli strain PK8277

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag KZY04_RS22995 Protein ID WP_001216045.1
Coordinates 19332..19712 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KZY04_RS23000 Protein ID WP_001190712.1
Coordinates 19712..19933 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY04_RS22970 (KZY04_22980) 15316..15690 + 375 WP_130541352.1 GNAT family N-acetyltransferase -
KZY04_RS22975 (KZY04_22985) 15719..16416 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
KZY04_RS22980 (KZY04_22990) 16534..17742 + 1209 WP_137922728.1 HAMP domain-containing methyl-accepting chemotaxis protein -
KZY04_RS22985 (KZY04_22995) 18077..19120 - 1044 WP_072647226.1 DUF968 domain-containing protein -
KZY04_RS22990 (KZY04_23000) 19148..19327 - 180 WP_000113018.1 hypothetical protein -
KZY04_RS22995 (KZY04_23005) 19332..19712 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KZY04_RS23000 (KZY04_23010) 19712..19933 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KZY04_RS23005 (KZY04_23015) 20116..21672 + 1557 WP_072647227.1 type I restriction-modification system subunit M -
KZY04_RS23010 (KZY04_23020) 21669..22937 + 1269 WP_072647224.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) - 1..98016 98016


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T211011 WP_001216045.1 NZ_CP080135:c19712-19332 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T211011 NZ_CP104660:2061775-2061877 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT211011 WP_001190712.1 NZ_CP080135:c19933-19712 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT211011 NZ_CP104660:c2061884-2061739 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References