Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 8899..9491 Replicon plasmid pPK8568-MCR1
Accession NZ_CP080130
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I9ZJC5
Locus tag KZY03_RS24655 Protein ID WP_000513532.1
Coordinates 8899..9147 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1I9ZJ49
Locus tag KZY03_RS24660 Protein ID WP_000681609.1
Coordinates 9144..9491 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS24620 (KZY03_24620) 3911..4576 - 666 WP_000532149.1 division plane positioning ATPase MipZ -
KZY03_RS24625 (KZY03_24625) 5165..5494 + 330 WP_001542022.1 hypothetical protein -
KZY03_RS24630 (KZY03_24630) 5895..6410 + 516 WP_001025393.1 J domain-containing protein -
KZY03_RS24635 (KZY03_24635) 6514..7056 + 543 WP_001083904.1 hypothetical protein -
KZY03_RS24640 (KZY03_24640) 7053..7307 + 255 WP_001230709.1 hypothetical protein -
KZY03_RS24645 (KZY03_24645) 7304..7546 + 243 WP_000151456.1 hypothetical protein -
KZY03_RS24650 (KZY03_24650) 8117..8410 + 294 WP_000673428.1 hypothetical protein -
KZY03_RS24655 (KZY03_24655) 8899..9147 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin Toxin
KZY03_RS24660 (KZY03_24660) 9144..9491 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KZY03_RS25225 9579..10022 - 444 WP_225930715.1 hypothetical protein -
KZY03_RS24670 (KZY03_24670) 10263..10562 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
KZY03_RS24675 (KZY03_24675) 10822..11148 - 327 WP_000206697.1 hypothetical protein -
KZY03_RS24680 (KZY03_24680) 11150..11473 - 324 WP_223666488.1 hypothetical protein -
KZY03_RS25230 11450..11590 - 141 Protein_19 hypothetical protein -
KZY03_RS24685 (KZY03_24685) 11610..12317 - 708 WP_000108794.1 hypothetical protein -
KZY03_RS24690 (KZY03_24690) 12319..13071 - 753 WP_000797966.1 conjugal transfer protein TraL -
KZY03_RS24695 (KZY03_24695) 13089..13490 - 402 WP_021575837.1 hypothetical protein -
KZY03_RS24700 (KZY03_24700) 13858..14199 + 342 WP_000730722.1 plasmid mobilization relaxosome protein MobC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..60659 60659


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9378.03 Da        Isoelectric Point: 10.4800

>T210989 WP_000513532.1 NZ_CP080130:8899-9147 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T210989 NZ_CP104651:522089-522475 [Acinetobacter variabilis]
ATGGATAATCGCTGTTTCAAGCTGAGACGCAGCAAGTGTGCGCTGGTGTTTCAGCTTTTTATTTTCGCTGTATTGATGTT
CTTGCTGTATCAGTTATTGCCTGTGACAATTTGGCTGGTTTGTGGCGTCATCGGATTGGTCATCTACCAGTTGTTTTATC
GTAAGACACCGCACATTGAGCAGTTTGAATATCTGGATGGACGTGAATGGTCACTGACTGCAGCTGCACAAGGCACACGG
CGAGTATTGATCAGTCACGTGATTGATCACCAAGCTTATATCGTGGTGTATTTCCAGCATGCAAAAGCCCGTCCATTGCT
CATCTGGTGTGACCAGTTGCCTTTTAAACAATGGAAGTCTTTGAAAGTTCTGACAAAAATACTTTAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13206.97 Da        Isoelectric Point: 4.8313

>AT210989 WP_000681609.1 NZ_CP080130:9144-9491 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Download         Length: 348 bp

>AT210989 NZ_CP104651:521839-522096 [Acinetobacter variabilis]
ATGACTGATGACATCACCTTAGAAGAGCGCAAAGTGATTTATCGCGCGCGTCGTGGCTTGAAAGAGATCGATGTATATTT
TGATCCCTACGTGAAAAACTATTATCTCAATGCTGATCCCTTCGAAAAAGAGATGTTTGCTGAGCTGGTTGCACAGGAAG
ATCCGGACCTGCTGGACTGGTTTATGGAAGTGTCAGAACCCCCACGCCCTGAATTAAAAGATTTTATCAAGAAGCTTAAA
TTTTACGTTCATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9ZJC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1I9ZJ49

References