Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 89523..90124 Replicon plasmid pPK8568-122kb
Accession NZ_CP080128
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag KZY03_RS23890 Protein ID WP_001216045.1
Coordinates 89744..90124 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KZY03_RS23885 Protein ID WP_001190712.1
Coordinates 89523..89744 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS23875 (KZY03_23875) 86531..87787 - 1257 WP_072196859.1 restriction endonuclease subunit S -
KZY03_RS23880 (KZY03_23880) 87784..89340 - 1557 WP_060578235.1 type I restriction-modification system subunit M -
KZY03_RS23885 (KZY03_23885) 89523..89744 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KZY03_RS23890 (KZY03_23890) 89744..90124 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KZY03_RS23895 (KZY03_23895) 90129..90308 + 180 WP_000113018.1 hypothetical protein -
KZY03_RS23900 (KZY03_23900) 90336..91379 + 1044 WP_130568730.1 DUF968 domain-containing protein -
KZY03_RS25160 91801..92151 + 351 Protein_94 ORF6N domain-containing protein -
KZY03_RS25165 92272..92604 + 333 WP_224205061.1 phage antirepressor KilAC domain-containing protein -
KZY03_RS23910 (KZY03_23910) 92637..93488 - 852 WP_000611664.1 phage repressor protein C1 -
KZY03_RS23915 (KZY03_23915) 93599..93808 - 210 WP_000874156.1 hypothetical protein -
KZY03_RS23920 (KZY03_23920) 93979..94676 + 698 WP_103215986.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / aph(3')-Ia / tet(A) - 1..122366 122366


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T210984 WP_001216045.1 NZ_CP080128:89744-90124 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T210984 NZ_CP104649:c4829412-4829194 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT210984 WP_001190712.1 NZ_CP080128:89523-89744 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT210984 NZ_CP104649:c4829812-4829438 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References