Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 73006..73528 Replicon plasmid pPK8568-122kb
Accession NZ_CP080128
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag KZY03_RS23800 Protein ID WP_000220560.1
Coordinates 73006..73287 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag KZY03_RS23805 Protein ID WP_000121743.1
Coordinates 73277..73528 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS23790 (KZY03_23790) 70227..71615 - 1389 WP_001207923.1 nitrate/nitrite transporter NarU -
KZY03_RS23795 (KZY03_23795) 71878..72858 + 981 WP_129753561.1 IS5-like element ISKpn26 family transposase -
KZY03_RS23800 (KZY03_23800) 73006..73287 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZY03_RS23805 (KZY03_23805) 73277..73528 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
KZY03_RS23810 (KZY03_23810) 74710..75678 + 969 WP_165403134.1 IS5-like element IS903B family transposase -
KZY03_RS23815 (KZY03_23815) 75838..75981 + 144 WP_152930453.1 hypothetical protein -
KZY03_RS23820 (KZY03_23820) 76042..76287 + 246 Protein_77 transposase -
KZY03_RS23825 (KZY03_23825) 76355..77507 + 1153 Protein_78 IS30-like element IS30 family transposase -
KZY03_RS23830 (KZY03_23830) 77520..77777 - 258 Protein_79 transposase -
KZY03_RS23835 (KZY03_23835) 77852..78163 + 312 Protein_80 VRR-NUC domain-containing protein -
KZY03_RS23840 (KZY03_23840) 78230..78385 + 156 WP_032236586.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / aph(3')-Ia / tet(A) - 1..122366 122366
- inside IScluster/Tn - - 71878..77507 5629


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T210983 WP_000220560.1 NZ_CP080128:c73287-73006 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T210983 NZ_CP104649:4570608-4571006 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT210983 WP_000121743.1 NZ_CP080128:c73528-73277 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT210983 NZ_CP104649:4570312-4570605 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References