Detailed information of TA system TA210979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 83875..84500
• Replicon: plasmid pPK8568-156kb
• Accession: NZ_CP080127
• Organism: Escherichia coli strain PK8568

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZY03_RS23005
• Protein ID: WP_000911324.1
• Coordinates: 84102..84500 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: KZY03_RS23000
• Protein ID: WP_000450532.1
• Coordinates: 83875..84102 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY03_RS23000 (83875)	83875..84102	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KZY03_RS23005 (84102)	84102..84500	+	399	WP_000911324.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZY03_RS23010 (84509)	84509..86662	-	2154	WP_000009350.1	type IV conjugative transfer system coupling protein TraD	-
KZY03_RS23015 (86915)	86915..87646	-	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
KZY03_RS23020 (87671)	87671..88192	-	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / blaTEM-1C / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN	1..156326	156326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T210979 WP_000911324.1 NZ_CP080127:84102-84500 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T210979 NZ_CP104649:3988373-3988546 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT210979 WP_000450532.1 NZ_CP080127:83875-84102 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT210979 NZ_CP104649:3988049-3988345 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5