Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 40397..41040 Replicon plasmid pPK8568-156kb
Accession NZ_CP080127
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag KZY03_RS22815 Protein ID WP_000754566.1
Coordinates 40624..41040 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag KZY03_RS22810 Protein ID WP_001261276.1
Coordinates 40397..40627 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS22775 (35598) 35598..35765 + 168 Protein_30 colicin-B -
KZY03_RS22780 (35783) 35783..36310 - 528 WP_000203272.1 colicin B immunity protein -
KZY03_RS22785 (36554) 36554..37369 + 816 WP_001312845.1 lipid II-degrading bacteriocin colicin M -
KZY03_RS22790 (37419) 37419..37772 - 354 WP_000864812.1 colicin M immunity protein -
KZY03_RS22795 (37950) 37950..38741 - 792 WP_000016493.1 site-specific integrase -
KZY03_RS22800 (38738) 38738..39427 - 690 WP_000796228.1 hypothetical protein -
KZY03_RS22805 (39471) 39471..39821 - 351 WP_000493379.1 hypothetical protein -
KZY03_RS22810 (40397) 40397..40627 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZY03_RS22815 (40624) 40624..41040 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
KZY03_RS22820 (41244) 41244..42100 + 857 Protein_39 IS3-like element ISEc15 family transposase -
KZY03_RS22825 (42382) 42382..43458 + 1077 WP_000227969.1 IS110 family transposase -
KZY03_RS22830 (43475) 43475..43753 + 279 Protein_41 IS3 family transposase -
KZY03_RS22835 (43861) 43861..45936 - 2076 WP_061350058.1 protease Lon-related BREX system protein BrxL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / tet(A) / blaTEM-1C / dfrA14 / sitABCD iroB / iroC / iroD / iroE / iroN 1..156326 156326


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T210978 WP_000754566.1 NZ_CP080127:40624-41040 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T210978 NZ_CP104649:c3251317-3251210 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT210978 WP_001261276.1 NZ_CP080127:40397-40627 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT210978 NZ_CP104649:3251365-3251431 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References