Detailed information of TA system TA210973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3496365..3497202
• Replicon: chromosome
• Accession: NZ_CP080126
• Organism: Escherichia coli strain PK8568

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KZY03_RS16960
• Protein ID: WP_000227784.1
• Coordinates: 3496660..3497202 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KZY03_RS16955
• Protein ID: WP_001297137.1
• Coordinates: 3496365..3496676 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY03_RS16930 (3491385)	3491385..3492332	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
KZY03_RS16935 (3492354)	3492354..3494345	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KZY03_RS16940 (3494335)	3494335..3494949	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KZY03_RS16945 (3494949)	3494949..3495278	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KZY03_RS16950 (3495290)	3495290..3496180	+	891	WP_000971336.1	heme o synthase	-
KZY03_RS16955 (3496365)	3496365..3496676	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KZY03_RS16960 (3496660)	3496660..3497202	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KZY03_RS16965 (3497258)	3497258..3498193	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
KZY03_RS16970 (3498601)	3498601..3499965	+	1365	WP_001000960.1	MFS transporter	-
KZY03_RS16975 (3500093)	3500093..3500584	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KZY03_RS16980 (3500752)	3500752..3501663	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T210973 WP_000227784.1 NZ_CP080126:3496660-3497202 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T210973 NZ_CP104649:c2783182-2782868 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT210973 WP_001297137.1 NZ_CP080126:3496365-3496676 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT210973 NZ_CP104649:c2782871-2782455 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3