Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3462288..3462906 Replicon chromosome
Accession NZ_CP080126
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZY03_RS16790 Protein ID WP_001291435.1
Coordinates 3462688..3462906 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZY03_RS16785 Protein ID WP_000344800.1
Coordinates 3462288..3462662 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS16775 (3457377) 3457377..3458570 + 1194 WP_219805021.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KZY03_RS16780 (3458593) 3458593..3461742 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KZY03_RS16785 (3462288) 3462288..3462662 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZY03_RS16790 (3462688) 3462688..3462906 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KZY03_RS16795 (3463078) 3463078..3463629 + 552 WP_000102564.1 maltose O-acetyltransferase -
KZY03_RS16800 (3463745) 3463745..3464215 + 471 WP_000136192.1 YlaC family protein -
KZY03_RS16805 (3464379) 3464379..3465929 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZY03_RS16810 (3465971) 3465971..3466324 - 354 WP_000878141.1 DUF1428 family protein -
KZY03_RS16820 (3466703) 3466703..3467014 + 312 WP_000409911.1 MGMT family protein -
KZY03_RS16825 (3467045) 3467045..3467617 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210972 WP_001291435.1 NZ_CP080126:3462688-3462906 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210972 NZ_CP104649:c2588067-2587660 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210972 WP_000344800.1 NZ_CP080126:3462288-3462662 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210972 NZ_CP104649:c2588314-2588048 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References