Detailed information of TA system TA210961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1315642..1316267
• Replicon: chromosome
• Accession: NZ_CP080126
• Organism: Escherichia coli strain PK8568

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZY03_RS06395
• Protein ID: WP_000911330.1
• Coordinates: 1315869..1316267 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KZY03_RS06390
• Protein ID: WP_000450524.1
• Coordinates: 1315642..1315869 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY03_RS06365 (1311445)	1311445..1311915	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KZY03_RS06370 (1311915)	1311915..1312487	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KZY03_RS06375 (1312633)	1312633..1313511	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KZY03_RS06380 (1313528)	1313528..1314562	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
KZY03_RS06385 (1314775)	1314775..1315488	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KZY03_RS06390 (1315642)	1315642..1315869	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KZY03_RS06395 (1315869)	1315869..1316267	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZY03_RS06400 (1316414)	1316414..1317277	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
KZY03_RS06405 (1317292)	1317292..1319307	+	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
KZY03_RS06410 (1319381)	1319381..1320079	+	699	WP_000679812.1	esterase	-
KZY03_RS06415 (1320160)	1320160..1320360	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T210961 WP_000911330.1 NZ_CP080126:1315869-1316267 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T210961 NZ_CP104647:c5335956-5335853 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT210961 WP_000450524.1 NZ_CP080126:1315642-1315869 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT210961 NZ_CP104647:5335815-5336086 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A829CM33