Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ypjF-yeeU/CbtA-CbeA
Location 1131397..1132129 Replicon chromosome
Accession NZ_CP080126
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name ypjF Uniprot ID A0A377CSV0
Locus tag KZY03_RS05525 Protein ID WP_001194227.1
Coordinates 1131397..1131726 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A377CUM4
Locus tag KZY03_RS05530 Protein ID WP_001059335.1
Coordinates 1131764..1132129 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS05505 (1126847) 1126847..1127824 - 978 WP_001315804.1 glutarate dioxygenase GlaH -
KZY03_RS05510 (1128160) 1128160..1129266 - 1107 Protein_1076 alpha amylase C-terminal domain-containing protein -
KZY03_RS05515 (1129333) 1129333..1130495 + 1163 WP_085947771.1 IS3-like element IS3 family transposase -
KZY03_RS05520 (1130523) 1130523..1130825 - 303 WP_000446741.1 hypothetical protein -
KZY03_RS05525 (1131397) 1131397..1131726 - 330 WP_001194227.1 TA system toxin CbtA family protein Toxin
KZY03_RS05530 (1131764) 1131764..1132129 - 366 WP_001059335.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KZY03_RS05535 (1132155) 1132155..1132376 - 222 WP_000691984.1 DUF987 domain-containing protein -
KZY03_RS05540 (1132373) 1132373..1132915 - 543 WP_001098974.1 DNA repair protein RadC -
KZY03_RS05545 (1132928) 1132928..1133371 - 444 WP_000381004.1 antirestriction protein -
KZY03_RS05550 (1133402) 1133402..1134223 - 822 WP_001234410.1 DUF932 domain-containing protein -
KZY03_RS05555 (1134315) 1134315..1134656 - 342 WP_000720953.1 hypothetical protein -
KZY03_RS05560 (1134691) 1134691..1135155 - 465 WP_000791791.1 hypothetical protein -
KZY03_RS05565 (1135281) 1135281..1135439 - 159 WP_001128372.1 hypothetical protein -
KZY03_RS05570 (1135661) 1135661..1136548 - 888 WP_001567853.1 50S ribosome-binding GTPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(17-117)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12445.11 Da        Isoelectric Point: 7.2767

>T210960 WP_001194227.1 NZ_CP080126:c1131726-1131397 [Escherichia coli]
MQTLSSHPTRATQPCLSPVETWQRLLTHLLSQHYGLTLNDTPFSNETTIREDIDAGVSLSDAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T210960 NZ_CP104647:5057399-5057677 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13545.40 Da        Isoelectric Point: 5.8806

>AT210960 WP_001059335.1 NZ_CP080126:c1132129-1131764 [Escherichia coli]
MNNHSVFGTKPENLTCQEWGLKRTITPCFGARLVQEGNRVHFLADRAGFNGAFIDDNALRLDLAFPLILKQLELMLTSGE
LSPRHEHCVTLYHNGLTCEADTLGSCGYVYIAIYPDQPEPH

Download         Length: 366 bp

>AT210960 NZ_CP104647:5057206-5057397 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377CSV0

Antitoxin

Source ID Structure
AlphaFold DB A0A377CUM4

References