Detailed information of TA system TA021096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1351676..1352595
• Replicon: chromosome
• Accession: NC_006322
• Organism: Bacillus licheniformis DSM 13 = ATCC 14580

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: BLI_RS06760
• Protein ID: WP_003180879.1
• Coordinates: 1351849..1352595 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: Q65L00
• Locus tag: BLI_RS06755
• Protein ID: WP_011197812.1
• Coordinates: 1351676..1351849 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLI_RS06735	1347101..1348198	+	1098	WP_003180867.1	mannonate dehydratase	-
BLI_RS06740	1348174..1349022	+	849	WP_009328728.1	SDR family oxidoreductase	-
BLI_RS06745	1349049..1350062	+	1014	WP_011197810.1	zinc-binding alcohol dehydrogenase family protein	-
BLI_RS06750	1350115..1351386	+	1272	WP_011197811.1	MFS transporter	-
BLI_RS06755	1351676..1351849	-	174	WP_011197812.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BLI_RS06760	1351849..1352595	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BLI_RS06765	1352706..1353704	-	999	WP_009328723.1	inorganic phosphate transporter	-
BLI_RS06770	1353717..1354334	-	618	WP_003180884.1	DUF47 domain-containing protein	-
BLI_RS06775	1354666..1356423	+	1758	WP_011197813.1	gamma-glutamyltransferase	-
BLI_RS06780	1356552..1357196	+	645	WP_009328721.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T21096 WP_003180879.1 NC_006322:c1352595-1351849 [Bacillus licheniformis DSM 13 = ATCC 14580]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T21096 NC_006322:c1352595-1351849 [Bacillus licheniformis DSM 13 = ATCC 14580]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTTATTGTTTTGGCGCTGGCTCTGTACGTAGCTGCCGTGTGGCGTTTTGA
AAAACAGCTCGCCGAAAAAACGGTCGCCATCAGAAAAACGTGGTACCTTCTTTATGTCATCGGCGCCGTGATATATTGGA
CGCACGATCCGCAGTCAATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGTTGACAGACGCTTTT
ATTTTCTTAAATGCCTACTTTAAAAAGCTCGGCAGCTCTGAACTCGCGACAGATACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
CCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6971.08 Da
• Isoelectric Point: 10.7815

>AT21096 WP_011197812.1 NC_006322:c1351849-1351676 [Bacillus licheniformis DSM 13 = ATCC 14580]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPYTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT21096 NC_006322:c1351849-1351676 [Bacillus licheniformis DSM 13 = ATCC 14580]
ATGGAGCCTGCTTTTCAGCAAGACCAGGAAAGCGTAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCTATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: Q65L00