Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 997138..997721 Replicon chromosome
Accession NZ_CP080126
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name mazF Uniprot ID F4NJF2
Locus tag KZY03_RS04845 Protein ID WP_000254731.1
Coordinates 997386..997721 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KZY03_RS04840 Protein ID WP_000581937.1
Coordinates 997138..997386 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS04830 (993477) 993477..994778 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KZY03_RS04835 (994826) 994826..997060 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KZY03_RS04840 (997138) 997138..997386 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KZY03_RS04845 (997386) 997386..997721 + 336 WP_000254731.1 endoribonuclease MazF Toxin
KZY03_RS04850 (997792) 997792..998583 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KZY03_RS04855 (998811) 998811..1000448 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KZY03_RS04860 (1000536) 1000536..1001834 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12043.02 Da        Isoelectric Point: 8.1170

>T210959 WP_000254731.1 NZ_CP080126:997386-997721 [Escherichia coli]
MVSRYVPDMGDLICVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Download         Length: 336 bp

>T210959 NZ_CP104647:c5005413-5005258 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT210959 WP_000581937.1 NZ_CP080126:997138-997386 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT210959 NZ_CP104647:5005425-5005483 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F4NJF2

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References