Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 288336..289136 Replicon chromosome
Accession NZ_CP080126
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KZY03_RS01325 Protein ID WP_000342449.1
Coordinates 288609..289136 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KZY03_RS01320 Protein ID WP_001277108.1
Coordinates 288336..288602 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS01300 (283993) 283993..284661 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KZY03_RS01305 (284654) 284654..285712 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KZY03_RS01310 (285957) 285957..286811 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KZY03_RS01315 (287083) 287083..288186 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KZY03_RS01320 (288336) 288336..288602 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KZY03_RS01325 (288609) 288609..289136 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KZY03_RS01330 (289133) 289133..289516 - 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
KZY03_RS01335 (289940) 289940..291049 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KZY03_RS01340 (291097) 291097..292023 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KZY03_RS01345 (292020) 292020..293297 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
KZY03_RS01350 (293294) 293294..294061 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T210956 WP_000342449.1 NZ_CP080126:288609-289136 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T210956 NZ_CP104647:c4585372-4585265 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT210956 WP_001277108.1 NZ_CP080126:288336-288602 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT210956 NZ_CP104647:4585425-4585486 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References