Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 267000..267631 Replicon chromosome
Accession NZ_CP080126
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag KZY03_RS01200 Protein ID WP_001259388.1
Coordinates 267000..267275 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KZY03_RS01205 Protein ID WP_000593555.1
Coordinates 267272..267631 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS01185 (262004) 262004..263071 + 1068 WP_000361485.1 HlyD family secretion protein -
KZY03_RS01190 (263068) 263068..265803 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
KZY03_RS01195 (265803) 265803..266927 + 1125 WP_001314210.1 ABC transporter permease -
KZY03_RS01200 (267000) 267000..267275 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
KZY03_RS01205 (267272) 267272..267631 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KZY03_RS01210 (267751) 267751..268152 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KZY03_RS01215 (268158) 268158..268964 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KZY03_RS01220 (268961) 268961..269725 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KZY03_RS01225 (269725) 269725..270558 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KZY03_RS01230 (270555) 270555..271499 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T210955 WP_001259388.1 NZ_CP080126:267000-267275 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T210955 NZ_CP104647:4348184-4348558 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT210955 WP_000593555.1 NZ_CP080126:267272-267631 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT210955 NZ_CP104647:4347763-4348137 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References