Detailed information of TA system TA210951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 74883..75484
• Replicon: plasmid pPK8217-139kb
• Accession: NZ_CP080123
• Organism: Escherichia coli strain PK8217

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: KZO72_RS23725
• Protein ID: WP_001216045.1
• Coordinates: 75104..75484 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: KZO72_RS23720
• Protein ID: WP_001190712.1
• Coordinates: 74883..75104 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO72_RS23695 (KZO72_23700)	70019..71218	+	1200	WP_000804064.1	tetracycline efflux MFS transporter Tet(A)	-
KZO72_RS23700 (KZO72_23705)	71250..72134	-	885	WP_000058717.1	EamA family transporter	-
KZO72_RS23705 (KZO72_23710)	72272..72664	-	393	WP_001351729.1	isochorismatase family cysteine hydrolase	-
KZO72_RS23710 (KZO72_23715)	72668..74419	+	1752	Protein_84	Tn3-like element TnAs1 family transposase	-
KZO72_RS23715 (KZO72_23720)	74455..74700	-	246	Protein_85	type I restriction-modification system subunit M N-terminal domain-containing protein	-
KZO72_RS23720 (KZO72_23725)	74883..75104	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KZO72_RS23725 (KZO72_23730)	75104..75484	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KZO72_RS23730 (KZO72_23735)	75489..75668	+	180	WP_024262170.1	hypothetical protein	-
KZO72_RS23735 (KZO72_23740)	75696..76739	+	1044	WP_072649708.1	DUF968 domain-containing protein	-
KZO72_RS23740 (KZO72_23745)	76828..77280	+	453	WP_001326849.1	late promoter-activating protein	-
KZO72_RS23745 (KZO72_23750)	77366..78559	+	1194	WP_064186818.1	hypothetical protein	-
KZO72_RS24845	78559..80043	+	1485	WP_232379031.1	terminase	-
KZO72_RS23750 (KZO72_23755)	80028..80429	+	402	WP_223663931.1	phage antirepressor KilAC domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / tet(A)	-	1..139269	139269
	-	inside	IScluster/Tn	blaTEM-1B / tet(A)	-	53642..74419	20777

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T210951 WP_001216045.1 NZ_CP080123:75104-75484 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T210951 NZ_CP104647:c3499996-3499778 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT210951 WP_001190712.1 NZ_CP080123:74883-75104 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT210951 NZ_CP104647:c3500396-3500022 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6