Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 54770..55292 Replicon plasmid pPK8217-139kb
Accession NZ_CP080123
Organism Escherichia coli strain PK8217

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag KZO72_RS23585 Protein ID WP_000220560.1
Coordinates 54770..55051 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag KZO72_RS23590 Protein ID WP_000121743.1
Coordinates 55041..55292 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO72_RS23575 (KZO72_23580) 51991..53379 - 1389 WP_001207923.1 nitrate/nitrite transporter NarU -
KZO72_RS23580 (KZO72_23585) 53642..54622 + 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
KZO72_RS23585 (KZO72_23590) 54770..55051 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZO72_RS23590 (KZO72_23595) 55041..55292 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
KZO72_RS23595 (KZO72_23600) 56452..57420 + 969 WP_000654812.1 IS5-like element IS903B family transposase -
KZO72_RS23600 (KZO72_23605) 57580..57723 + 144 WP_152930453.1 hypothetical protein -
KZO72_RS23605 (KZO72_23610) 57784..58029 + 246 Protein_63 transposase -
KZO72_RS23610 (KZO72_23615) 58097..59248 + 1152 WP_219808370.1 IS30-like element IS30 family transposase -
KZO72_RS23615 (KZO72_23620) 59261..59518 - 258 Protein_65 transposase -
KZO72_RS23620 (KZO72_23625) 59593..59904 + 312 Protein_66 VRR-NUC domain-containing protein -
KZO72_RS23625 (KZO72_23630) 59971..60126 + 156 WP_032236586.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / tet(A) - 1..139269 139269
- inside IScluster/Tn blaTEM-1B / tet(A) - 53642..74419 20777


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T210950 WP_000220560.1 NZ_CP080123:c55051-54770 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T210950 NZ_CP104647:3241195-3241593 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT210950 WP_000121743.1 NZ_CP080123:c55292-55041 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT210950 NZ_CP104647:3240899-3241192 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References