Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 90175..91798 Replicon plasmid pPK8217-MCR1
Accession NZ_CP080122
Organism Escherichia coli strain PK8217

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KZO72_RS22335 Protein ID WP_000088046.1
Coordinates 90473..91798 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KZO72_RS22330 Protein ID WP_024136327.1
Coordinates 90175..90468 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO72_RS25040 85245..85376 + 132 WP_000374059.1 hypothetical protein -
KZO72_RS22300 (KZO72_22290) 85717..85998 - 282 WP_000077926.1 hypothetical protein -
KZO72_RS22305 (KZO72_22295) 86048..86239 - 192 WP_000398480.1 hypothetical protein -
KZO72_RS22310 (KZO72_22300) 86463..87635 + 1173 WP_000952349.1 IS21-like element IS21 family transposase -
KZO72_RS22315 (KZO72_22305) 87635..88432 + 798 WP_000544828.1 IS21-like element IS21 family helper ATPase IstB -
KZO72_RS22320 (KZO72_22310) 88471..88806 - 336 WP_065311125.1 hypothetical protein -
KZO72_RS22325 (KZO72_22315) 89179..89571 + 393 WP_000341066.1 hypothetical protein -
KZO72_RS22330 (KZO72_22320) 90175..90468 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KZO72_RS22335 (KZO72_22325) 90473..91798 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KZO72_RS22340 (KZO72_22330) 91859..92065 + 207 WP_000134171.1 hypothetical protein -
KZO72_RS22345 (KZO72_22335) 92167..92577 + 411 WP_000985911.1 hypothetical protein -
KZO72_RS22350 (KZO72_22340) 92590..93405 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
KZO72_RS22355 (KZO72_22345) 93659..94084 + 426 WP_001043843.1 hypothetical protein -
KZO72_RS22360 (KZO72_22350) 94404..94610 - 207 WP_127762864.1 hypothetical protein -
KZO72_RS22365 (KZO72_22355) 94633..94941 + 309 WP_001224687.1 hypothetical protein -
KZO72_RS22370 (KZO72_22360) 94957..95814 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KZO72_RS22375 (KZO72_22365) 95876..96079 + 204 WP_001194555.1 hypothetical protein -
KZO72_RS22380 (KZO72_22370) 96421..96777 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-1.1 / aph(3')-Ia / ant(3'')-Ia / lnu(F) / sul3 / tet(A) / aph(6)-Id / floR / ARR-3 / dfrA14 - 1..267091 267091


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T210949 WP_000088046.1 NZ_CP080122:90473-91798 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T210949 NZ_CP104647:3003507-3003821 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT210949 WP_024136327.1 NZ_CP080122:90175-90468 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT210949 NZ_CP104647:3003271-3003504 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References