Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3319335..3319953 Replicon chromosome
Accession NZ_CP080121
Organism Escherichia coli strain PK8217

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZO72_RS16190 Protein ID WP_001291435.1
Coordinates 3319735..3319953 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZO72_RS16185 Protein ID WP_000344800.1
Coordinates 3319335..3319709 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO72_RS16175 (3314424) 3314424..3315617 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KZO72_RS16180 (3315640) 3315640..3318789 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KZO72_RS16185 (3319335) 3319335..3319709 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZO72_RS16190 (3319735) 3319735..3319953 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KZO72_RS16195 (3320125) 3320125..3320676 + 552 WP_219794986.1 maltose O-acetyltransferase -
KZO72_RS16200 (3320792) 3320792..3321262 + 471 WP_000136192.1 YlaC family protein -
KZO72_RS16205 (3321426) 3321426..3322976 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZO72_RS16210 (3323018) 3323018..3323371 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KZO72_RS16220 (3323750) 3323750..3324061 + 312 WP_000409911.1 MGMT family protein -
KZO72_RS16225 (3324092) 3324092..3324664 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210945 WP_001291435.1 NZ_CP080121:3319735-3319953 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210945 NZ_CP104647:c1921880-1921773 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210945 WP_000344800.1 NZ_CP080121:3319335-3319709 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210945 NZ_CP104647:1921928-1921994 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References