Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 825172..825826 Replicon chromosome
Accession NZ_CP080121
Organism Escherichia coli strain PK8217

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KZO72_RS04060 Protein ID WP_000244777.1
Coordinates 825419..825826 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZO72_RS04055 Protein ID WP_000354046.1
Coordinates 825172..825438 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO72_RS04030 (820341) 820341..821084 + 744 WP_000951962.1 SDR family oxidoreductase -
KZO72_RS04035 (821141) 821141..822574 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
KZO72_RS04040 (822619) 822619..822930 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KZO72_RS04045 (823094) 823094..823753 + 660 WP_000250274.1 hemolysin III family protein -
KZO72_RS04050 (823949) 823949..824929 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZO72_RS04055 (825172) 825172..825438 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZO72_RS04060 (825419) 825419..825826 + 408 WP_000244777.1 protein YgfX Toxin
KZO72_RS04065 (825866) 825866..826387 - 522 WP_001055874.1 flavodoxin FldB -
KZO72_RS04070 (826499) 826499..827395 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZO72_RS04075 (827420) 827420..828130 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZO72_RS04080 (828136) 828136..829869 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T210932 WP_000244777.1 NZ_CP080121:825419-825826 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210932 NZ_CP104645:4825559-4825735 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210932 WP_000354046.1 NZ_CP080121:825172-825438 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210932 NZ_CP104645:4825781-4826197 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References