Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 244414..245056 Replicon chromosome
Accession NC_006274
Organism Bacillus cereus E33L

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BCE33L_RS01370 Protein ID WP_000635963.1
Coordinates 244706..245056 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BCE33L_RS01365 Protein ID WP_000004570.1
Coordinates 244414..244701 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCE33L_RS01340 239730..240692 + 963 WP_000961148.1 UV DNA damage repair endonuclease UvsE -
BCE33L_RS01345 240685..241257 - 573 WP_000906925.1 rhomboid family intramembrane serine protease -
BCE33L_RS01350 241350..241709 + 360 WP_000635039.1 holo-ACP synthase -
BCE33L_RS01355 241866..242816 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BCE33L_RS01360 242935..244104 + 1170 WP_000390606.1 alanine racemase -
BCE33L_RS01365 244414..244701 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BCE33L_RS01370 244706..245056 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BCE33L_RS01375 245124..247292 + 2169 WP_000426226.1 RNA-binding transcriptional accessory protein -
BCE33L_RS01380 247351..247467 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BCE33L_RS01385 247663..248121 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T21093 WP_000635963.1 NC_006274:244706-245056 [Bacillus cereus E33L]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T21093 NC_006274:244706-245056 [Bacillus cereus E33L]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT21093 WP_000004570.1 NC_006274:244414-244701 [Bacillus cereus E33L]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT21093 NC_006274:244414-244701 [Bacillus cereus E33L]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References