Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4585178..4586013 Replicon chromosome
Accession NZ_CP080120
Organism Escherichia coli strain M24

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag KZX72_RS22335 Protein ID WP_000854759.1
Coordinates 4585178..4585555 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag KZX72_RS22340 Protein ID WP_001295723.1
Coordinates 4585645..4586013 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX72_RS22310 (4581289) 4581289..4582911 - 1623 WP_001295726.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -
KZX72_RS22315 (4583702) 4583702..4583878 - 177 Protein_4407 helix-turn-helix domain-containing protein -
KZX72_RS22320 (4584245) 4584245..4584394 - 150 Protein_4408 hypothetical protein -
KZX72_RS22325 (4584500) 4584500..4584676 - 177 WP_000839286.1 DUF957 domain-containing protein -
KZX72_RS22330 (4584693) 4584693..4585181 - 489 WP_000761690.1 DUF5983 family protein -
KZX72_RS22335 (4585178) 4585178..4585555 - 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
KZX72_RS22340 (4585645) 4585645..4586013 - 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KZX72_RS22345 (4586286) 4586286..4587032 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
KZX72_RS22350 (4587047) 4587047..4588588 - 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
KZX72_RS22355 (4588729) 4588729..4588983 - 255 WP_023281719.1 DUF987 domain-containing protein -
KZX72_RS22360 (4589046) 4589046..4589522 - 477 WP_001186775.1 RadC family protein -
KZX72_RS22365 (4589538) 4589538..4590011 - 474 WP_001350782.1 antirestriction protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T210925 WP_000854759.1 NZ_CP080120:c4585555-4585178 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T210925 NZ_CP104645:c3484140-3483922 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT210925 WP_001295723.1 NZ_CP080120:c4586013-4585645 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT210925 NZ_CP104645:c3484540-3484166 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References