Detailed information of TA system TA210925

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4585178..4586013
• Replicon: chromosome
• Accession: NZ_CP080120
• Organism: Escherichia coli strain M24

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: KZX72_RS22335
• Protein ID: WP_000854759.1
• Coordinates: 4585178..4585555 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: KZX72_RS22340
• Protein ID: WP_001295723.1
• Coordinates: 4585645..4586013 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX72_RS22310 (4581289)	4581289..4582911	-	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
KZX72_RS22315 (4583702)	4583702..4583878	-	177	Protein_4407	helix-turn-helix domain-containing protein	-
KZX72_RS22320 (4584245)	4584245..4584394	-	150	Protein_4408	hypothetical protein	-
KZX72_RS22325 (4584500)	4584500..4584676	-	177	WP_000839286.1	DUF957 domain-containing protein	-
KZX72_RS22330 (4584693)	4584693..4585181	-	489	WP_000761690.1	DUF5983 family protein	-
KZX72_RS22335 (4585178)	4585178..4585555	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
KZX72_RS22340 (4585645)	4585645..4586013	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZX72_RS22345 (4586286)	4586286..4587032	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
KZX72_RS22350 (4587047)	4587047..4588588	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
KZX72_RS22355 (4588729)	4588729..4588983	-	255	WP_023281719.1	DUF987 domain-containing protein	-
KZX72_RS22360 (4589046)	4589046..4589522	-	477	WP_001186775.1	RadC family protein	-
KZX72_RS22365 (4589538)	4589538..4590011	-	474	WP_001350782.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T210925 WP_000854759.1 NZ_CP080120:c4585555-4585178 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T210925 NZ_CP104645:c3484140-3483922 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT210925 WP_001295723.1 NZ_CP080120:c4586013-4585645 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT210925 NZ_CP104645:c3484540-3484166 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52