Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4394387..4394907 Replicon chromosome
Accession NZ_CP080120
Organism Escherichia coli strain M24

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag KZX72_RS21420 Protein ID WP_001160964.1
Coordinates 4394387..4394671 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KZX72_RS21425 Protein ID WP_000125566.1
Coordinates 4394674..4394907 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX72_RS21395 (4390032) 4390032..4391318 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
KZX72_RS21400 (4391318) 4391318..4392307 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KZX72_RS21405 (4392304) 4392304..4393125 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KZX72_RS21410 (4393128) 4393128..4393505 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KZX72_RS21415 (4393512) 4393512..4394360 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KZX72_RS21420 (4394387) 4394387..4394671 - 285 WP_001160964.1 CcdB family protein Toxin
KZX72_RS21425 (4394674) 4394674..4394907 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KZX72_RS21430 (4394993) 4394993..4395472 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
KZX72_RS21435 (4395664) 4395664..4397526 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
KZX72_RS21440 (4397519) 4397519..4398049 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KZX72_RS21445 (4398157) 4398157..4399488 - 1332 WP_001183209.1 MFS transporter -
KZX72_RS21450 (4399547) 4399547..4399834 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T210923 WP_001160964.1 NZ_CP080120:c4394671-4394387 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T210923 NZ_CP104645:2988943-2989257 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT210923 WP_000125566.1 NZ_CP080120:c4394907-4394674 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT210923 NZ_CP104645:2988707-2988940 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References