Detailed information of TA system TA210922

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4056509..4057310
• Replicon: chromosome
• Accession: NZ_CP080120
• Organism: Escherichia coli strain M24

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KZX72_RS19845
• Protein ID: WP_061091468.1
• Coordinates: 4056509..4056886 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KZX72_RS19850
• Protein ID: WP_032215284.1
• Coordinates: 4056933..4057310 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX72_RS19810 (4052445)	4052445..4052732	-	288	Protein_3915	DUF4942 domain-containing protein	-
KZX72_RS19815 (4053014)	4053014..4054018	-	1005	WP_001179707.1	IS110-like element ISSfl8 family transposase	-
KZX72_RS19820 (4054090)	4054090..4054650	-	561	Protein_3917	DUF4942 domain-containing protein	-
KZX72_RS19825 (4054747)	4054747..4054944	-	198	WP_061091469.1	DUF957 domain-containing protein	-
KZX72_RS19830 (4054964)	4054964..4055113	-	150	Protein_3919	DUF5983 family protein	-
KZX72_RS19835 (4055193)	4055193..4056110	+	918	WP_000520337.1	IS5-like element ISEc35 family transposase	-
KZX72_RS19840 (4056084)	4056084..4056512	-	429	Protein_3921	DUF5983 family protein	-
KZX72_RS19845 (4056509)	4056509..4056886	-	378	WP_061091468.1	TA system toxin CbtA family protein	Toxin
KZX72_RS19850 (4056933)	4056933..4057310	-	378	WP_032215284.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZX72_RS19855 (4057473)	4057473..4057694	-	222	WP_000692307.1	DUF987 domain-containing protein	-
KZX72_RS19860 (4057763)	4057763..4058239	-	477	WP_001186774.1	RadC family protein	-
KZX72_RS19865 (4058255)	4058255..4058728	-	474	WP_000855069.1	antirestriction protein	-
KZX72_RS19870 (4058822)	4058822..4059067	-	246	WP_000680586.1	hypothetical protein	-
KZX72_RS19875 (4059067)	4059067..4059888	-	822	WP_061091467.1	DUF932 domain-containing protein	-
KZX72_RS19880 (4060256)	4060256..4060738	-	483	WP_077777324.1	hypothetical protein	-
KZX72_RS19885 (4061099)	4061099..4061641	-	543	WP_033811096.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14223.17 Da
• Isoelectric Point: 6.7029

>T210922 WP_061091468.1 NZ_CP080120:c4056886-4056509 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTLFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T210922 NZ_CP104645:c2873090-2872749 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13806.71 Da
• Isoelectric Point: 6.7387

>AT210922 WP_032215284.1 NZ_CP080120:c4057310-4056933 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT210922 NZ_CP104645:2873085-2873161 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT