210921

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3869210..3869828 Replicon chromosome
Accession NZ_CP080120
Organism Escherichia coli strain M24

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZX72_RS18955 Protein ID WP_001291435.1
Coordinates 3869610..3869828 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZX72_RS18950 Protein ID WP_000344800.1
Coordinates 3869210..3869584 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX72_RS18940 (3864299) 3864299..3865492 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KZX72_RS18945 (3865515) 3865515..3868664 + 3150 WP_001132478.1 efflux RND transporter permease AcrB -
KZX72_RS18950 (3869210) 3869210..3869584 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZX72_RS18955 (3869610) 3869610..3869828 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KZX72_RS18960 (3870002) 3870002..3870553 + 552 WP_000102539.1 maltose O-acetyltransferase -
KZX72_RS18965 (3870669) 3870669..3871139 + 471 WP_000136192.1 YlaC family protein -
KZX72_RS18970 (3871303) 3871303..3872853 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZX72_RS18975 (3872895) 3872895..3873248 - 354 WP_000878135.1 DUF1428 family protein -
KZX72_RS18985 (3873627) 3873627..3873938 + 312 WP_000409908.1 MGMT family protein -
KZX72_RS18990 (3873969) 3873969..3874541 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210921 WP_001291435.1 NZ_CP080120:3869610-3869828 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210921 NZ_CP104645:2739876-2740226 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210921 WP_000344800.1 NZ_CP080120:3869210-3869584 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210921 NZ_CP104645:2739631-2739882 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References