210903

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 397811..398611 Replicon chromosome
Accession NZ_CP080120
Organism Escherichia coli strain M24

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag KZX72_RS01845 Protein ID WP_000342452.1
Coordinates 398084..398611 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag KZX72_RS01840 Protein ID WP_001277107.1
Coordinates 397811..398077 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX72_RS01820 (393470) 393470..394138 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KZX72_RS01825 (394131) 394131..395189 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
KZX72_RS01830 (395434) 395434..396288 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KZX72_RS01835 (396559) 396559..397662 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KZX72_RS01840 (397811) 397811..398077 + 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KZX72_RS01845 (398084) 398084..398611 + 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KZX72_RS01850 (398608) 398608..398991 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
KZX72_RS01855 (399414) 399414..400523 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KZX72_RS01860 (400571) 400571..401497 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KZX72_RS01865 (401494) 401494..402771 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
KZX72_RS01870 (402768) 402768..403535 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T210903 WP_000342452.1 NZ_CP080120:398084-398611 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T210903 NZ_CP104643:c2789659-2789504 [Salmonella enterica]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT210903 WP_001277107.1 NZ_CP080120:397811-398077 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT210903 NZ_CP104643:2789671-2789729 [Salmonella enterica]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References