Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2343399..2343919 Replicon chromosome
Accession NZ_CP080119
Organism Escherichia coli strain M45

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag KZX76_RS11250 Protein ID WP_001160964.1
Coordinates 2343635..2343919 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KZX76_RS11245 Protein ID WP_000125566.1
Coordinates 2343399..2343632 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX76_RS11220 (2338472) 2338472..2338759 + 288 WP_000203744.1 ferredoxin-like protein FixX -
KZX76_RS11225 (2338818) 2338818..2340149 + 1332 WP_001183209.1 MFS transporter -
KZX76_RS11230 (2340257) 2340257..2340787 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KZX76_RS11235 (2340780) 2340780..2342642 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
KZX76_RS11240 (2342834) 2342834..2343313 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
KZX76_RS11245 (2343399) 2343399..2343632 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KZX76_RS11250 (2343635) 2343635..2343919 + 285 WP_001160964.1 CcdB family protein Toxin
KZX76_RS11255 (2343946) 2343946..2344794 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KZX76_RS11260 (2344801) 2344801..2345178 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KZX76_RS11265 (2345181) 2345181..2346002 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KZX76_RS11270 (2345999) 2345999..2346988 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KZX76_RS11275 (2346988) 2346988..2348274 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T210883 WP_001160964.1 NZ_CP080119:2343635-2343919 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T210883 NZ_CP104641:c989152-988625 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT210883 WP_000125566.1 NZ_CP080119:2343399-2343632 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT210883 NZ_CP104641:c989439-989149 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References