Detailed information of TA system TA210881

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2107512..2108347
• Replicon: chromosome
• Accession: NZ_CP080119
• Organism: Escherichia coli strain M45

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: KZX76_RS10075
• Protein ID: WP_000854759.1
• Coordinates: 2107970..2108347 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: KZX76_RS10070
• Protein ID: WP_001295723.1
• Coordinates: 2107512..2107880 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX76_RS10045 (2103518)	2103518..2103991	+	474	WP_049143783.1	antirestriction protein	-
KZX76_RS10050 (2104003)	2104003..2104479	+	477	WP_122997348.1	RadC family protein	-
KZX76_RS10055 (2104542)	2104542..2104796	+	255	WP_023281719.1	DUF987 domain-containing protein	-
KZX76_RS10060 (2104937)	2104937..2106478	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
KZX76_RS10065 (2106493)	2106493..2107239	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
KZX76_RS10070 (2107512)	2107512..2107880	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZX76_RS10075 (2107970)	2107970..2108347	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
KZX76_RS10080 (2108344)	2108344..2108832	+	489	WP_000761690.1	DUF5983 family protein	-
KZX76_RS10085 (2108849)	2108849..2109025	+	177	WP_000839286.1	DUF957 domain-containing protein	-
KZX76_RS10090 (2109131)	2109131..2109280	+	150	Protein_1971	hypothetical protein	-
KZX76_RS10095 (2109647)	2109647..2109823	+	177	Protein_1972	helix-turn-helix domain-containing protein	-
KZX76_RS10100 (2110614)	2110614..2112236	+	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T210881 WP_000854759.1 NZ_CP080119:2107970-2108347 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T210881 NZ_CP104641:825270-825683 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT210881 WP_001295723.1 NZ_CP080119:2107512-2107880 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT210881 NZ_CP104641:825023-825289 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52