Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 994806..995606 Replicon chromosome
Accession NZ_CP080119
Organism Escherichia coli strain M45

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag KZX76_RS04845 Protein ID WP_000342452.1
Coordinates 994806..995333 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag KZX76_RS04850 Protein ID WP_001277107.1
Coordinates 995340..995606 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX76_RS04820 (989882) 989882..990649 - 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KZX76_RS04825 (990646) 990646..991923 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
KZX76_RS04830 (991920) 991920..992846 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KZX76_RS04835 (992894) 992894..994003 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KZX76_RS04840 (994426) 994426..994809 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
KZX76_RS04845 (994806) 994806..995333 - 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KZX76_RS04850 (995340) 995340..995606 - 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KZX76_RS04855 (995755) 995755..996858 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KZX76_RS04860 (997129) 997129..997983 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KZX76_RS04865 (998228) 998228..999286 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
KZX76_RS04870 (999279) 999279..999947 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T210876 WP_000342452.1 NZ_CP080119:c995333-994806 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T210876 NZ_CP104640:19268-19573 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT210876 WP_001277107.1 NZ_CP080119:c995606-995340 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT210876 NZ_CP104640:19048-19266 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References