210870

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5087615..5088191 Replicon chromosome
Accession NZ_CP080118
Organism Escherichia coli strain M70

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag KZX75_RS24660 Protein ID WP_001295743.1
Coordinates 5087904..5088191 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag KZX75_RS24655 Protein ID WP_000063148.1
Coordinates 5087615..5087917 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX75_RS24640 (5084211) 5084211..5086361 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KZX75_RS24645 (5086411) 5086411..5086614 + 204 WP_000467859.1 YbdD/YjiX family protein -
KZX75_RS24650 (5086625) 5086625..5087581 + 957 WP_001295745.1 GTPase -
KZX75_RS24655 (5087615) 5087615..5087917 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
KZX75_RS24660 (5087904) 5087904..5088191 - 288 WP_001295743.1 BrnT family toxin Toxin
KZX75_RS24665 (5088388) 5088388..5089554 + 1167 WP_000800831.1 restriction endonuclease -
KZX75_RS24670 (5089618) 5089618..5091237 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
KZX75_RS24675 (5091227) 5091227..5092531 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T210870 WP_001295743.1 NZ_CP080118:c5088191-5087904 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T210870 NZ_CP104639:c4500616-4500509 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT210870 WP_000063148.1 NZ_CP080118:c5087917-5087615 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT210870 NZ_CP104639:4500669-4500730 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References