Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4977629..4978149 Replicon chromosome
Accession NZ_CP080118
Organism Escherichia coli strain M70

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag KZX75_RS24130 Protein ID WP_001160964.1
Coordinates 4977629..4977913 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KZX75_RS24135 Protein ID WP_000125566.1
Coordinates 4977916..4978149 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX75_RS24105 (4973274) 4973274..4974560 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
KZX75_RS24110 (4974560) 4974560..4975549 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KZX75_RS24115 (4975546) 4975546..4976367 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KZX75_RS24120 (4976370) 4976370..4976747 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KZX75_RS24125 (4976754) 4976754..4977602 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KZX75_RS24130 (4977629) 4977629..4977913 - 285 WP_001160964.1 CcdB family protein Toxin
KZX75_RS24135 (4977916) 4977916..4978149 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KZX75_RS24140 (4978235) 4978235..4978714 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
KZX75_RS24145 (4978906) 4978906..4980768 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
KZX75_RS24150 (4980761) 4980761..4981291 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KZX75_RS24155 (4981399) 4981399..4982730 - 1332 WP_001183209.1 MFS transporter -
KZX75_RS24160 (4982789) 4982789..4983076 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T210869 WP_001160964.1 NZ_CP080118:c4977913-4977629 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T210869 NZ_CP104639:4242642-4243016 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT210869 WP_000125566.1 NZ_CP080118:c4978149-4977916 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT210869 NZ_CP104639:4242221-4242595 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References