Detailed information of TA system TA210868

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4641164..4641965
• Replicon: chromosome
• Accession: NZ_CP080118
• Organism: Escherichia coli strain M70

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KZX75_RS22575
• Protein ID: WP_061091468.1
• Coordinates: 4641164..4641541 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KZX75_RS22580
• Protein ID: WP_032215284.1
• Coordinates: 4641588..4641965 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX75_RS22540 (4637100)	4637100..4637387	-	288	Protein_4438	DUF4942 domain-containing protein	-
KZX75_RS22545 (4637669)	4637669..4638673	-	1005	WP_001179707.1	IS110-like element ISSfl8 family transposase	-
KZX75_RS22550 (4638745)	4638745..4639305	-	561	Protein_4440	DUF4942 domain-containing protein	-
KZX75_RS22555 (4639402)	4639402..4639599	-	198	WP_061091469.1	DUF957 domain-containing protein	-
KZX75_RS22560 (4639619)	4639619..4639768	-	150	Protein_4442	DUF5983 family protein	-
KZX75_RS22565 (4639848)	4639848..4640765	+	918	WP_000520337.1	IS5-like element ISEc35 family transposase	-
KZX75_RS22570 (4640739)	4640739..4641167	-	429	Protein_4444	DUF5983 family protein	-
KZX75_RS22575 (4641164)	4641164..4641541	-	378	WP_061091468.1	TA system toxin CbtA family protein	Toxin
KZX75_RS22580 (4641588)	4641588..4641965	-	378	WP_032215284.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZX75_RS22585 (4642128)	4642128..4642349	-	222	WP_000692307.1	DUF987 domain-containing protein	-
KZX75_RS22590 (4642418)	4642418..4642894	-	477	WP_001186774.1	RadC family protein	-
KZX75_RS22595 (4642910)	4642910..4643383	-	474	WP_000855069.1	antirestriction protein	-
KZX75_RS22600 (4643477)	4643477..4643722	-	246	WP_000680586.1	hypothetical protein	-
KZX75_RS22605 (4643722)	4643722..4644543	-	822	WP_061091467.1	DUF932 domain-containing protein	-
KZX75_RS22610 (4644911)	4644911..4645393	-	483	WP_077777324.1	hypothetical protein	-
KZX75_RS22615 (4645754)	4645754..4646296	-	543	WP_033811096.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4594837..4665827	70990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14223.17 Da
• Isoelectric Point: 6.7029

>T210868 WP_061091468.1 NZ_CP080118:c4641541-4641164 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTLFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T210868 NZ_CP104639:c3955687-3955400 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13806.71 Da
• Isoelectric Point: 6.7387

>AT210868 WP_032215284.1 NZ_CP080118:c4641965-4641588 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT210868 NZ_CP104639:c3955878-3955687 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA