210867

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4451746..4452364 Replicon chromosome
Accession NZ_CP080118
Organism Escherichia coli strain M70

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZX75_RS21670 Protein ID WP_001291435.1
Coordinates 4452146..4452364 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZX75_RS21665 Protein ID WP_000344800.1
Coordinates 4451746..4452120 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX75_RS21655 (4446835) 4446835..4448028 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KZX75_RS21660 (4448051) 4448051..4451200 + 3150 WP_001132478.1 efflux RND transporter permease AcrB -
KZX75_RS21665 (4451746) 4451746..4452120 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZX75_RS21670 (4452146) 4452146..4452364 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KZX75_RS21675 (4452538) 4452538..4453089 + 552 WP_000102539.1 maltose O-acetyltransferase -
KZX75_RS21680 (4453205) 4453205..4453675 + 471 WP_000136192.1 YlaC family protein -
KZX75_RS21685 (4453839) 4453839..4455389 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZX75_RS21690 (4455431) 4455431..4455784 - 354 WP_000878135.1 DUF1428 family protein -
KZX75_RS21700 (4456163) 4456163..4456474 + 312 WP_000409908.1 MGMT family protein -
KZX75_RS21705 (4456505) 4456505..4457077 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210867 WP_001291435.1 NZ_CP080118:4452146-4452364 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210867 NZ_CP104639:c3789715-3789329 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210867 WP_000344800.1 NZ_CP080118:4451746-4452120 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210867 NZ_CP104639:c3789339-3789010 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References