Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1745335..1745989 Replicon chromosome
Accession NZ_CP080118
Organism Escherichia coli strain M70

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag KZX75_RS08325 Protein ID WP_000244765.1
Coordinates 1745582..1745989 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag KZX75_RS08320 Protein ID WP_000354050.1
Coordinates 1745335..1745601 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX75_RS08300 (1741423) 1741423..1742856 - 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -
KZX75_RS08305 (1742901) 1742901..1743212 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
KZX75_RS08310 (1743376) 1743376..1744035 + 660 WP_000250275.1 hemolysin III family protein -
KZX75_RS08315 (1744112) 1744112..1745092 - 981 WP_000886084.1 tRNA-modifying protein YgfZ -
KZX75_RS08320 (1745335) 1745335..1745601 + 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
KZX75_RS08325 (1745582) 1745582..1745989 + 408 WP_000244765.1 protein YgfX Toxin
KZX75_RS08330 (1746029) 1746029..1746550 - 522 WP_001055874.1 flavodoxin FldB -
KZX75_RS08335 (1746662) 1746662..1747558 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZX75_RS08340 (1747583) 1747583..1748293 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZX75_RS08345 (1748299) 1748299..1750032 + 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T210854 WP_000244765.1 NZ_CP080118:1745582-1745989 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210854 NZ_CP104639:c1109148-1108741 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT210854 WP_000354050.1 NZ_CP080118:1745335-1745601 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210854 NZ_CP104639:c1109395-1109129 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References