Detailed information of TA system TA210853

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1598818..1599652
• Replicon: chromosome
• Accession: NZ_CP080118
• Organism: Escherichia coli strain M70

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: KZX75_RS07570
• Protein ID: WP_000854689.1
• Coordinates: 1598818..1599195 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1X3JHN3
• Locus tag: KZX75_RS07575
• Protein ID: WP_001285598.1
• Coordinates: 1599272..1599652 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX75_RS07540 (1595198)	1595198..1595368	-	171	Protein_1476	IS110 family transposase	-
KZX75_RS07545 (1595839)	1595839..1596732	-	894	WP_001114681.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
KZX75_RS07550 (1596725)	1596725..1597120	-	396	WP_000208383.1	DUF6088 family protein	-
KZX75_RS07555 (1597189)	1597189..1598034	-	846	WP_023281696.1	DUF4942 domain-containing protein	-
KZX75_RS07560 (1598119)	1598119..1598316	-	198	WP_000839293.1	DUF957 domain-containing protein	-
KZX75_RS07565 (1598333)	1598333..1598821	-	489	WP_000761699.1	DUF5983 family protein	-
KZX75_RS07570 (1598818)	1598818..1599195	-	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
KZX75_RS07575 (1599272)	1599272..1599652	-	381	WP_001285598.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZX75_RS07580 (1599702)	1599702..1600346	-	645	WP_000094916.1	hypothetical protein	-
KZX75_RS07585 (1600365)	1600365..1600586	-	222	WP_000692329.1	DUF987 domain-containing protein	-
KZX75_RS07590 (1600649)	1600649..1601125	-	477	WP_001186726.1	RadC family protein	-
KZX75_RS07595 (1601141)	1601141..1601626	-	486	WP_000849566.1	antirestriction protein	-
KZX75_RS07600 (1601681)	1601681..1602499	-	819	WP_001234620.1	DUF932 domain-containing protein	-
KZX75_RS07605 (1602600)	1602600..1602809	-	210	WP_032142237.1	DUF905 family protein	-
KZX75_RS07610 (1602914)	1602914..1603369	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T210853 WP_000854689.1 NZ_CP080118:c1599195-1598818 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T210853 NZ_CP104639:c963666-963331 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13927.72 Da
• Isoelectric Point: 4.7959

>AT210853 WP_001285598.1 NZ_CP080118:c1599652-1599272 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT210853 NZ_CP104639:c963914-963666 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1X3JHN3