Detailed information of TA system TA210849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 1067669..1068469
• Replicon: chromosome
• Accession: NZ_CP080118
• Organism: Escherichia coli strain M70

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: KZX75_RS04955
• Protein ID: WP_000342452.1
• Coordinates: 1067942..1068469 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: KZX75_RS04950
• Protein ID: WP_001277107.1
• Coordinates: 1067669..1067935 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX75_RS04930 (1063328)	1063328..1063996	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
KZX75_RS04935 (1063989)	1063989..1065047	+	1059	WP_001042013.1	permease-like cell division protein FtsX	-
KZX75_RS04940 (1065292)	1065292..1066146	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
KZX75_RS04945 (1066417)	1066417..1067520	+	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
KZX75_RS04950 (1067669)	1067669..1067935	+	267	WP_001277107.1	type II toxin-antitoxin system antitoxin AtaR	Antitoxin
KZX75_RS04955 (1067942)	1067942..1068469	+	528	WP_000342452.1	type II toxin-antitoxin system toxin acetyltransferase AtaT	Toxin
KZX75_RS04960 (1068466)	1068466..1068849	-	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
KZX75_RS04965 (1069272)	1069272..1070381	+	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
KZX75_RS04970 (1070429)	1070429..1071355	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KZX75_RS04975 (1071352)	1071352..1072629	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
KZX75_RS04980 (1072626)	1072626..1073393	+	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T210849 WP_000342452.1 NZ_CP080118:1067942-1068469 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T210849 NZ_CP104638:3959167-3959535 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAACAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT210849 WP_001277107.1 NZ_CP080118:1067669-1067935 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT210849 NZ_CP104638:3958949-3959170 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTAACGGCGGGCCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57