Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4215699..4216275 Replicon chromosome
Accession NZ_CP080117
Organism Escherichia coli strain OV24

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag KZX74_RS20575 Protein ID WP_001295743.1
Coordinates 4215988..4216275 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag KZX74_RS20570 Protein ID WP_000063148.1
Coordinates 4215699..4216001 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX74_RS20555 (4212295) 4212295..4214445 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KZX74_RS20560 (4214495) 4214495..4214698 + 204 WP_000467859.1 YbdD/YjiX family protein -
KZX74_RS20565 (4214709) 4214709..4215665 + 957 WP_001295745.1 GTPase -
KZX74_RS20570 (4215699) 4215699..4216001 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
KZX74_RS20575 (4215988) 4215988..4216275 - 288 WP_001295743.1 BrnT family toxin Toxin
KZX74_RS20580 (4216472) 4216472..4217638 + 1167 WP_000800831.1 restriction endonuclease -
KZX74_RS20585 (4217702) 4217702..4219321 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
KZX74_RS20590 (4219311) 4219311..4220615 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T210840 WP_001295743.1 NZ_CP080117:c4216275-4215988 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T210840 NZ_CP104633:c10746-10495 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATCACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGAAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT210840 WP_000063148.1 NZ_CP080117:c4216001-4215699 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT210840 NZ_CP104633:c10508-10281 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References