Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4105712..4106232 Replicon chromosome
Accession NZ_CP080117
Organism Escherichia coli strain OV24

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag KZX74_RS20045 Protein ID WP_001160964.1
Coordinates 4105712..4105996 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KZX74_RS20050 Protein ID WP_000125566.1
Coordinates 4105999..4106232 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX74_RS20020 (4101357) 4101357..4102643 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
KZX74_RS20025 (4102643) 4102643..4103632 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KZX74_RS20030 (4103629) 4103629..4104450 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KZX74_RS20035 (4104453) 4104453..4104830 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KZX74_RS20040 (4104837) 4104837..4105685 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KZX74_RS20045 (4105712) 4105712..4105996 - 285 WP_001160964.1 CcdB family protein Toxin
KZX74_RS20050 (4105999) 4105999..4106232 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KZX74_RS20055 (4106318) 4106318..4106797 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
KZX74_RS20060 (4106989) 4106989..4108851 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
KZX74_RS20065 (4108844) 4108844..4109374 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KZX74_RS20070 (4109482) 4109482..4110813 - 1332 WP_001183209.1 MFS transporter -
KZX74_RS20075 (4110872) 4110872..4111159 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T210839 WP_001160964.1 NZ_CP080117:c4105996-4105712 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T210839 NZ_CP104632:c13621-13343 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT210839 WP_000125566.1 NZ_CP080117:c4106232-4105999 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT210839 NZ_CP104632:c13884-13624 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References