Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3654802..3655420 Replicon chromosome
Accession NZ_CP080117
Organism Escherichia coli strain OV24

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZX74_RS17895 Protein ID WP_001291435.1
Coordinates 3655202..3655420 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZX74_RS17890 Protein ID WP_000344800.1
Coordinates 3654802..3655176 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX74_RS17880 (3649891) 3649891..3651084 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KZX74_RS17885 (3651107) 3651107..3654256 + 3150 WP_001132478.1 efflux RND transporter permease AcrB -
KZX74_RS17890 (3654802) 3654802..3655176 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZX74_RS17895 (3655202) 3655202..3655420 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KZX74_RS17900 (3655594) 3655594..3656145 + 552 WP_000102539.1 maltose O-acetyltransferase -
KZX74_RS17905 (3656261) 3656261..3656731 + 471 WP_000136192.1 YlaC family protein -
KZX74_RS17910 (3656895) 3656895..3658445 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZX74_RS17915 (3658487) 3658487..3658840 - 354 WP_000878135.1 DUF1428 family protein -
KZX74_RS17925 (3659219) 3659219..3659530 + 312 WP_000409908.1 MGMT family protein -
KZX74_RS17930 (3659561) 3659561..3660133 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210837 WP_001291435.1 NZ_CP080117:3655202-3655420 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210837 NZ_CP104629:4666664-4666978 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210837 WP_000344800.1 NZ_CP080117:3654802-3655176 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210837 NZ_CP104629:4666428-4666661 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGCGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References