Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 988162..988816 Replicon chromosome
Accession NZ_CP080117
Organism Escherichia coli strain OV24

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag KZX74_RS04820 Protein ID WP_000244765.1
Coordinates 988409..988816 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag KZX74_RS04815 Protein ID WP_000354050.1
Coordinates 988162..988428 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX74_RS04795 (984250) 984250..985683 - 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -
KZX74_RS04800 (985728) 985728..986039 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
KZX74_RS04805 (986203) 986203..986862 + 660 WP_000250275.1 hemolysin III family protein -
KZX74_RS04810 (986939) 986939..987919 - 981 WP_000886084.1 tRNA-modifying protein YgfZ -
KZX74_RS04815 (988162) 988162..988428 + 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
KZX74_RS04820 (988409) 988409..988816 + 408 WP_000244765.1 protein YgfX Toxin
KZX74_RS04825 (988856) 988856..989377 - 522 WP_001055874.1 flavodoxin FldB -
KZX74_RS04830 (989489) 989489..990385 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZX74_RS04835 (990410) 990410..991120 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZX74_RS04840 (991126) 991126..992859 + 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T210824 WP_000244765.1 NZ_CP080117:988409-988816 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210824 NZ_CP104629:c1830929-1830678 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT210824 WP_000354050.1 NZ_CP080117:988162-988428 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210824 NZ_CP104629:c1830681-1830427 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References