Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 397808..398608 Replicon chromosome
Accession NZ_CP080117
Organism Escherichia coli strain OV24

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag KZX74_RS01850 Protein ID WP_000342452.1
Coordinates 398081..398608 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag KZX74_RS01845 Protein ID WP_001277107.1
Coordinates 397808..398074 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX74_RS01825 (393467) 393467..394135 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KZX74_RS01830 (394128) 394128..395186 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
KZX74_RS01835 (395431) 395431..396285 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KZX74_RS01840 (396556) 396556..397659 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KZX74_RS01845 (397808) 397808..398074 + 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KZX74_RS01850 (398081) 398081..398608 + 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KZX74_RS01855 (398605) 398605..398988 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
KZX74_RS01860 (399411) 399411..400520 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KZX74_RS01865 (400568) 400568..401494 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KZX74_RS01870 (401491) 401491..402768 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
KZX74_RS01875 (402765) 402765..403532 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T210821 WP_000342452.1 NZ_CP080117:398081-398608 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T210821 NZ_CP104629:c1317527-1316205 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT210821 WP_001277107.1 NZ_CP080117:397808-398074 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT210821 NZ_CP104629:c1317793-1317527 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References