Detailed information of TA system TA210804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2700777..2701577
• Replicon: chromosome
• Accession: NZ_CP080116
• Organism: Escherichia coli strain Dog168

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: KZX73_RS13175
• Protein ID: WP_000342452.1
• Coordinates: 2701050..2701577 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: KZX73_RS13170
• Protein ID: WP_001277107.1
• Coordinates: 2700777..2701043 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX73_RS13150 (2696436)	2696436..2697104	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
KZX73_RS13155 (2697097)	2697097..2698155	+	1059	WP_001042013.1	permease-like cell division protein FtsX	-
KZX73_RS13160 (2698400)	2698400..2699254	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
KZX73_RS13165 (2699525)	2699525..2700628	+	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
KZX73_RS13170 (2700777)	2700777..2701043	+	267	WP_001277107.1	type II toxin-antitoxin system antitoxin AtaR	Antitoxin
KZX73_RS13175 (2701050)	2701050..2701577	+	528	WP_000342452.1	type II toxin-antitoxin system toxin acetyltransferase AtaT	Toxin
KZX73_RS13180 (2701574)	2701574..2701957	-	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
KZX73_RS13185 (2702380)	2702380..2703489	+	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
KZX73_RS13190 (2703537)	2703537..2704463	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KZX73_RS13195 (2704460)	2704460..2705737	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
KZX73_RS13200 (2705734)	2705734..2706501	+	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T210804 WP_000342452.1 NZ_CP080116:2701050-2701577 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T210804 NZ_CP104622:12338-12598 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT210804 WP_001277107.1 NZ_CP080116:2700777-2701043 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT210804 NZ_CP104622:12588-12839 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57