Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 1715938..1716533 Replicon chromosome
Accession NZ_CP080116
Organism Escherichia coli strain Dog168

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag KZX73_RS08625 Protein ID WP_000239579.1
Coordinates 1715938..1716288 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag KZX73_RS08630 Protein ID WP_001223208.1
Coordinates 1716282..1716533 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX73_RS08605 (1711384) 1711384..1712406 - 1023 WP_001296689.1 ABC transporter permease -
KZX73_RS08610 (1712420) 1712420..1713922 - 1503 WP_000205813.1 sugar ABC transporter ATP-binding protein -
KZX73_RS08615 (1714062) 1714062..1715018 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KZX73_RS08620 (1715328) 1715328..1715858 + 531 WP_000055075.1 inorganic diphosphatase -
KZX73_RS08625 (1715938) 1715938..1716288 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
KZX73_RS08630 (1716282) 1716282..1716533 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KZX73_RS08635 (1716745) 1716745..1717086 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KZX73_RS08640 (1717089) 1717089..1720868 - 3780 WP_000060945.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T210800 WP_000239579.1 NZ_CP080116:c1716288-1715938 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T210800 NZ_CP104620:2072-2359 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GGAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT210800 WP_001223208.1 NZ_CP080116:c1716533-1716282 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT210800 NZ_CP104620:1824-2075 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAGCTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGTCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References